Tag: Protein

Calcium-Dependent Actin Filament: DISCUSSION(9)

In summary, this study analyses the changes in the concentration and localization of the actin filament-severing protein scinderin, during testicular development, and in spermatozoa during and following the epididymal transit. Immunoblot analyses showed one band at 80 kDa in chromaffin cells, fetal and adult tubules, interstitial cells, aorta and vena cava, and spermatozoa. Scinderin immunoreactiv-ity […]

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Calcium-Dependent Actin Filament: DISCUSSION(8)

The changes reported here in the distribution of the Ca2+-dependent actin filament-severing protein scinder-in in the spermatozoa during and following epididymal transit may represent a different response of the gametes to local conditions that altered the [Ca2+]i. Scinderin is a calcium-dependent actin filament-severing protein that has been shown to have a major role in the […]

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Calcium-Dependent Actin Filament: DISCUSSION(7)

ScinderininEpididymalCells Scinderin immunoreactivity in epididymal cells also coincides with the presence of the cortical perijunctional actin filament network reported along the principal cells’ lateral plasma membranes. Whether scinderin accompanies the cell junctions in the epididymal cells or in the Sertoli cells, the protein may conceivably have similar effects on cellular contacts.

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Calcium-Dependent Actin Filament: DISCUSSION(6)

The sequestration of [Ca2+]i in the cisternae of ER accompanying the cortical or peripheral actin in the Sertoli cells could induce modifications in affinity of the Ca2+-dependent actin-interacting protein scin-derin for either the cytosol, the phospholipids of the membrane, or actin; these modifications, in turn, could prove significant in the physiology of the Sertoli cell […]

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Calcium-Dependent Actin Filament: DISCUSSION(5)

In Sertoli cells. In the testis, Sertoli cells share a morphological feature found in cells of most epithelia: they possess a layer of subsurface or cortical actin that occupies the peripheral cytoplasm and surrounds the perimeter of the cell. In the Sertoli cells, this cortical or peripheral actin is typically sandwiched between cisternae of endoplasmic […]

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Calcium-Dependent Actin Filament: DISCUSSION(4)

Actin, particularly F-actin, has been identified within the subacrosomal space of developing spermatids. Collectively, these authors emphasize that F-actin decreases with the completion of the spermatid’s development until it becomes virtually undetectable by the time mature spermatids are released from the seminiferous tubules. This suggests that the older the spermatid, the more abundant the G-actin […]

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Calcium-Dependent Actin Filament: DISCUSSION(3)

The present work is the first to document the variations in the concentration and the localization of the Ca2+-depen-dent actin filament-severing protein scinderin or adseverin, which reportedly shares a similar sequence, in testicular and epididymal cells during development, and in spermatozoa during and after their epididymal transit. It is also the first successful attempt to […]

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