Category: Sodium Chloride

Rat Oocytes Fertilized in Modified Rat 1-Cell Embryo: DISCUSSION(3)

In the present studies, we demonstrated successful development to term after transfer of morulae and early blastocysts that were fertilized in mR1ECM-BSA containing 110 mM NaCl and developed in a chemically defined medium, mR1ECM. However, the litter size was very small, and more than half the recipients had failed to maintain the pregnancy around Days […]

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Rat Oocytes Fertilized in Modified Rat 1-Cell Embryo: DISCUSSION(2)

Currently, more than 80% of 1-cell rat embryos can develop to the blastocyst stage in a chemically defined mR1ECM. However, the developmental ability of 1-cell rat embryos in mR1ECM is known to be very low when obtained soon after penetration in vitro. This phenomenon has also been described in hamster embryos in a chemically defined […]

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Rat Oocytes Fertilized in Modified Rat 1-Cell Embryo: DISCUSSION(1)

In the present studies, we demonstrated that successful sperm penetration was achieved in mR1ECM with modifications in which PVA was replaced with BSA and the NaCl concentration was increased to 100-130 mM. In hamsters, mice, and cattle, sperm penetration of oocytes in vitro was achieved when cumulus-oocyte complexes were cocultured in media not containing BSA. […]

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Rat Oocytes Fertilized in Modified Rat 1-Cell Embryo: RESULTS(2)

Optimal Concentration of NaCl in mR1ECM-BSA for Sperm Penetration (Experiment 3) Table 5 shows the proportion of oocytes penetrated during coculture with spermatozoa in mR1ECM-BSA containing various concentrations (90-140 mM) of NaCl for 10 h. The incidence of oocytes penetrated was high, and no differences were noted (p > 0.05) among mR1ECM-BSA medium containing 100-130 […]

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Rat Oocytes Fertilized in Modified Rat 1-Cell Embryo: RESULTS(1)

Effect of BSA in mR1ECM (Experiment 1) The incidence of oocytes penetrated during coculture of oocyte-cumulus complexes with spermatozoa in mKRB, mKRB-PVA, mR1ECM, and mR1ECM-BSA for 10 h is shown in Table 2. Sperm penetration was observed only in mKRB (88.2 ± 2.7%). Although no sperm penetration occurred in mR1ECM-BSA, this medium was used in […]

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Rat Oocytes Fertilized in Modified Rat 1-Cell Embryo: MATERIALS AND METHODS(5)

After coculture of cumulus-oocyte complexes with spermatozoa in the above-mentioned modified mR1ECM-BSA for 10 h, the incidence of sperm penetration was compared to that in mKRB. Further, to examine the effect on sperm penetration of supplementation to adjust osmolarity, the osmolarity of mR1ECM-BSA was increased to the same level (310 mOsm) in mKRB by increasing […]

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