Category: Ovariectomized

Time-Course of the Uterine Response to Estradiol: DISCUSSION(4)

However, in the present study, microvascular volume density increased nearly 2fold from 0 to 24 h. Thus, our data indicate that vasodilation probably accounts for a significant portion of the uterine microvascular response to E2, which is consistent with its well-known role as a powerful stimulator of uterine blood flow. It is highly unlikely, however, […]

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Time-Course of the Uterine Response to Estradiol: DISCUSSION(3)

In the mouse myometrium, however, nuclear incorporation of [3H]thymidine increased only slightly, and numbers of cells did not change, even though myo-metrial volume increased dramatically. Thus, in OVX, E2-treated mice, the response of the myometrium was due almost entirely to increased cell size, whereas in sheep, both increased cell proliferation and cell size contributed to […]

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Time-Course of the Uterine Response to Estradiol: DISCUSSION(2)

In the present study, the uterine growth response was due primarily to tissue growth (hyperplasia and hypertrophy) rather than to a change in the ratio of tissue dry weight:fresh weight. In addition, tissue growth was due approximately equally to hyperplasia and hypertrophy during the initial 24 h after E2 treatment. The initial uterine growth response […]

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Time-Course of the Uterine Response to Estradiol: DISCUSSION(1)

Although uterine responses to E2 have been investigated in OVX rodents, rabbits, primates, and ruminants, we are not aware of any study in which uterine growth, cell proliferation, and microvascular development have been evaluated in the same animals. In addition, because of the importance of steroid-mediated uterine responses during pregnancy and also in pathological conditions […]

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Time-Course of the Uterine Response to Estradiol: RESULTS(4)

By 24 h, however, moderate (glandular epithelium) to extensive (luminal epithelium and stroma) BrdU incorporation was observed in the endometrium; BrdU labeling in the myometrium, however, was still low (Table 4). At 48 h and 72 h, a relatively large proportion of the cells exhibited nuclear incorporation of BrdU, indicating a relatively high rate of […]

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Time-Course of the Uterine Response to Estradiol: RESULTS(3)

The further dramatic increase in uterine size that occurred between 24 h and 48 h (Table 1), however, was due primarily to increased cell size, since both the RNA:DNA and protein: DNA ratios increased (p < 0.01), whereas uterine DNA content remained constant (Table 3). In addition, the cellular hypertrophy was reflected by cellular morphology, […]

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Time-Course of the Uterine Response to Estradiol: RESULTS(2)

The cross-sectional areas of the uterine lumen, endometrium, and myometrium all increased (p < 0.01) from 0 to 72 h (Table 2). In addition, across all groups the crosssectional area of the endometrium was less (p < 0.01) than that of the myometrium (Table 2). The endometrium and myometrium remained constant proportions (p > 0.10) […]

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