Luteal Regression in the Normally Cycling Rat: MATERIALS AND METHODS(2)

MATERIALS AND METHODS(2)

From the remaining ovary, specific populations or sets of corpora lutea were dissected: on proestrus, the corpora lutea of the current cycle; on estrus, the regressing corpora lutea from the preceding cycle. Once dissected, the corpora lutea from each rat were counted and weighed as a group. antibiotics levaquin

In experiment 2, rats were killed on either the morning (0900-1000 h) of proestrus (n = 7) or the afternoon (16001800 h) of estrus (n = 7) as described above, and the ovaries were removed and placed on ice. Specific sets of corpora lutea were dissected from both ovaries: on proestrus, the corpora lutea of the current cycle; on estrus, the regressing corpora lutea from the preceding cycle. The dissected corpora lutea (n = 5-8) from one ovary were frozen as a group for sectioning; the sections were used for im-munohistochemistry for monocytes/macrophages, differentiated macrophages, and MCP-1 protein and for in situ detection of apoptosis. The remaining dissected corpora lutea from each rat were counted and weighed as a group.

In experiment 3, rats were killed on either the morning or early afternoon (1000-1300 h) of proestrus (n = 10) or on the afternoon (1500-1700 h) of estrus (n = 10). Rats were killed as described for experiment 1 and the ovaries removed and placed on ice. Corpora lutea of the current cycle (proestrus) or regressing corpora lutea from the preceding cycle (estrus) were dissected from both ovaries, weighed, frozen in liquid nitrogen, and stored at -80°C for later analysis of total protein content.

Category: Protein

Tags: Apoptosis, Inflammatory Cell, Luteal Regression, Monocyte Chemoattractant Protein, Protein

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