Calcium-Dependent Actin Filament-Severing Protein Scinderin Levels and Localization in Bovine Testis, Epididymis, and Spermatozoa(1)
Actin has been identified in both Sertoli cells and germ cells, namely, in developing spermatids and spermatozoa. ampicillin antibiotic
In the testis, impermeable tight junctions accompanied by microfilaments are situated between Sertoli cells, where they form the anatomical basis of the blood-testis barrier that divides the seminiferous epithelium into a basal and a luminal cellular compartment. At a precise moment of their development, germ cells located in the basal compartment must migrate into the luminal compartment to complete their meiosis and undergo cellular differentiation.
Exactly how this event takes place remains the subject of numerous scientific investigations. We recently presented data that suggested the control of the monomeric (G-actin) or filamentous (F-actin) state or form of the perijunctional actin may affect the association of the tight junction peripheral protein ZO-1 with other junctional components of the plasma membrane and perhaps in this way play a role in the regulation of the tight junction permeability. Because the state of the perijunctional actin is in part controlled by actin-binding proteins, in the study reported here, we elected to evaluate the variations in the concentration and localization of scinderin, a Ca2+-dependent actin filament-severing protein during testicular development.