No MTR transcripts were detected if water replaced the cDNA template in the PCR reaction. The lack of a positive signal in those prostate samples pretreated with RNase prior to the PCR reaction (data not shown) indicated that any PCR products arose from cDNA. Specificity was confirmed by sequence analysis and hybridization with a gene-specific internal oligonucleotide probe. Strong positive signals were observed in the myometrium, prostate, and epididymis of the adult tammar compared with weaker signals in equivalent tissues in the juvenile and pouch young males (Fig. 2b). A weak positive signal was also present in the testis. Cheap Diskus Advair
Localization of MTR Transcripts in the Prostate
The tammar prostate surrounds the urethra as it leaves the bladder and consists of numerous tubular glands arranged concentrically around the urethra (Fig. 3a). A dense layer of smooth muscle separates the prostatic glands from the urethral lumen (Fig. 3b). Smooth muscle cells are also present in the stromal tissue between the prostatic glands (Fig. 3c). These glands are lined with columnar epithelial cells that vary in height between prostate segments; the lumina contain secretory material with small globular bodies (Fig. 3c). The outermost layer of the prostate is a fi-broelastic capsule with smooth muscle fibers on the inner aspect. Blood vessels are present in this outer connective tissue layer (Fig. 3d). MTR transcripts were identified in the smooth muscle layer surrounding the urethral lumen (Fig. 4a) and also in the outer fibroelastic capsule. There was no evidence of MTR mRNA in the stromal tissue between the glands, but there was some positive staining in the basal part of the glandular epithelium (Fig. 4b). Sections that were hybridized with the sense probe, or pretreated with RNase before hybridization, showed no positive signals, and there were no specific MTR transcripts in the testis (Fig. 4c).
FIG. 3. Histology of the adult tammar prostate (hematoxylin- and eosin-stained sections). a, b) Numerous tubular glands (G) are arranged concentrically around the urethral lumen (UL), with a dense layer of smooth muscle (SM) between the prostatic glands and urethral lumen. c) Smooth muscle cells (arrowheads) are present in the stromal tissue between the prostatic glands, which are lined with columnar epithelial cells (EC). d) The outermost layer of the prostate is a fibroelastic capsule (FC) containing smooth muscle fibers and blood vessels (V). a) X7.5; b) X50; c) X100; d) X62.5 (reproduced at 70%).
FIG. 4. Localization of MTR transcripts using in situ hybridization with a digoxigenin-labeled MTR antisense probe. a, b) MTR transcripts (arrowheads) are present in the smooth muscle layer surrounding the lumen and also in the basal part of the glandular epithelium. c) No positive signals are evident in the adult testis. a, b) X50; c) X25 (reproduced at 68%).