Uterine Environment and Breed: RESULTS(2)

RESULTS(2)

Hematological Measures: Fetal

Fetal RBC increased (p < 0.01) on both Day 30 and Day 40 (Table 3) and did not differ according to treatment. Fetal hematocrit percentage increased (p < 0.01) on both Day 30 and Day 40 and was higher (p < 0.01) in WC than in MS (Table 3). Fetal hemoglobin concentrations increased {p < 0.01) on Day 30 and Day 40 (Table 3) and did not differ with different treatments. EPO levels were higher (p< 0.01) on Day 30 than on Day 40 and were higher (p < 0.01) in WC than in MS. buy flovent inhaler

Regression analysis revealed no significant relationship between fetal weight and hematocrit or fetal weight and hemoglobin on Day 40 in either WC INT or MS (intact uterine environment groups), which did not differ. A positive (p < 0.05) linear relationship was obtained between these traits in UHO, which differed (p < 0.05) from the INT and MS groups combined.

Ematological Measures: Maternal

Total maternal RBC were higher on Day 40 in INT than in UHO {p < 0.05) or MS (p < 0.01; Table 4). Maternal hematocrit percentage was higher in INT than in UHO (p< 0.05) on Day 24 and in WC than in MS on both Day 30 (p < 0.01) and Day 40 (p < 0.01; Table 4). Maternal hemoglobin concentrations were higher (p < 0.01) on Day 24 in INT than in UHO or MS (Table 4). Maternal plasma EPO concentrations were higher (p < 0.01) on Day 40 than on Day 30 (Table 4) and did not differ for treatments.

Differential Cell Counts of Erythroid Precursors in Fetal Blood

The percentages of BE and PE decreased (p < 0.01) with day of gestation, and PE was higher (p < 0.01) in WC than in MS (Table 5). Percentage of OE was higher (p < 0.01) on Day 30 than on Day 24 or Day 40 and was higher (p< 0.01) in MS or UHO than in INT on Day 24 (Table 5); it did not differ for treatments. Erythrocyte percentage increased (p < 0.05) with day of gestation and was higher (p < 0.01) on Day 40 in MS than in WC (Table 5). Protein 4, AFP, and RBP were unaffected by day or treatment.

Two-Dimensional PAGE Analysis of Fetal Liver Protein Secretion

The ratio of TF to total counts increased with day of gestation (p < 0.01) and was higher (p < 0.05) in WC than in MS (Table 6). Protein 5 ratio was higher (p < 0.01) on Day 30 or Day 40 than on Day 24 (Table 6).
TABLE 4.
Table4Uterine environment
a Least-squares means ± SEM; number of pregnant gilts in parentheses.
b Total RBC numbers were greater in INT than in UHO (p < 0.05) or MS (p < 0.01) on Day 40.
c Percent hematocrit was greater (p < 0.05) in INT than in UHO on Day 24 and was greater (p < 0.01) in WC than in MS on both Day 30 and Day 40.
d Hemoglobin concentrations were greater (p < 0.01) on Day 24 in INT vs. UHO or MS. e EPO (plasma erythropoietin) levels were greater (p < 0.01) on Day 40 than on Day 30.

TABLE 5.
Table5Uterine environment
Least-squares means 2: tor percentage ot erythroid precursor cell populations; number of pregnant gilts are in parentheses. b The percentage of basophilic erythroblasts decreased (p < 0.01) with age. c The percentage of polychromatic erythroblasts decreased (p < 0.01) with age.
d The percentage of orthochromatic erythroblasts was greater (p < 0.01) on Day 30 than on Day 24 or Day 40 and was greater (p < 0 01) in MS or UHO than in INT on Day 24.
e No significant effects of age or treatment were observed for reticulocyte percentage.
‘The percentage of erythrocytes increased (p < 0.01) with age and was greater (p < 0.01) on Day 40 in MS than in WC.

TABLE 6.
Table6Uterine environment
a Least-squares means ± SEM; data are expressed as the ratio of radioactivity contained in individual protein spots punched from 2-D gels to the total amount of radioactivity contained in nondialyzable macromolecules added to the gel; number of pregnant gilts in parentheses. b The ratio of transferrin increased (p < 0.01) with age and was greater (p < 0.05) in WC than in MS. c No significant effects of day or treatment were observed for alpha-fetoprotein. d No significant effects of day or treatment were observed for retinol-binding protein. e No significant effects of day or treatment were observed for protein 4.
‘ Protein 5 ratio was greater (p < 0.01) on Day 30 or Day 40 than on Day 24.

About

So Many Advances in Medicine, So Many Yet to Come