Significant differences (due to day of gestation) were observed for all cell types except reticulocytes. As expected, the more “primitive” precursors, BE and PE, were more prevalent on Day 24. OE were highest on Day 30, whereas erythrocytes were highest on Day 40. MS fetuses had a significantly lower percentage of PE, a more primitive precursor, than did WC, but a higher percentage of OE on Day 24, and a higher percentage of erythrocytes on Day 40 (Table 5). These data suggest that MS fetuses may produce more mature erythroid cells earlier in gestation than do WC fetuses. Further, WC fetuses had higher hematocrits than MS, despite no statistical difference in total circulating RBC between the two breeds. The smaller volume of the more mature erythroid precursors in the MS fetus could contribute to this observation. buy asthma inhalers
Two-Dimensional PACE Analysis of Fetal Liver Protein Secretion
Data from the 2D-PAGE analysis indicate that the secretion of TF and protein 5 by the liver are both increasing at this critical time for fetal erythropoiesis (Table 6). Transferrin is an iron transport protein in the pig fetus and is important (along with uteroferrin) in supplying this mineral to developing erythropoietic and other tissues. Total iron in the fetal liver increases between Day 30 and Day 45 in the pig, as does iron in allantoic fluid, where uteroferrin is metabolized and gives up iron to TF. The significance of the overall higher ratio of TF in WC than in MS is unknown at this time. Protein 5 has yet to be identified, and further characterization will be required to ascertain its potential role in porcine fetal erythropoiesis. Alpha-fetoprotein was chosen for analysis in this study since it is a major secreted liver protein in the fetus during the time period studied and appeared to have a constant level of expression, as was observed.