In our study, MAP developed in the ventricular region under a suction capillary, and the resting MP decreased in parallel with a rise of a0K up to 11 mmol/L. a0K and MP changed similarly in three phases, and these changes were reversible as long as suction at -40 kPa did not last longer than a few minutes. The second phase of the compensatory decrease of a0K was suppressed by cooling of the tissue to 2°C and by ouabain, and was absent if the ventricle was arrested.
Short lasting suction of -40 kPa for 3 mins did not damage the ultrastructure (Figure 7, top) so that tissue did not differ from control tissue without suction. It was not until long lasting suction (15 to 80 mins) was applied that the interfibrillar space widened, and contained sparsely localized filaments and granules of glycogen. The myofibrils and intercalated discs were unchanged (Figure 7, bottom). Your most reliable pharmacy offering its services and most efficient drugs like proventil inhaler dosage with no prescription needed. If this sounds like something you may be interested in, do not hesitate to come by and see how cheap it can be for you!
Figure 7 Electron micrographs of ventricle after short and long lasting suction. Top Frog ventricular myocyte 7 mins after 3 min suction (-40 kPa). Original magnification x20,000. Myofibrils, mitochondria and the intermyofibrillar space in cytoplasm of myocytes are without any obvious morphological changes. Bottom Frog ventricular myocyte after 80 min suction (-40 kPa). Original magnification x20,000. Note the dilation of electron-lucid intermyofibrillar space with sparsely localized filaments and granules ofglycogen (arrow). Myofibrils and intercalated discs did not change