Influence of the phospholipid n-6:n-3 polyunsaturated fatty acid ratio on the oxidative phosphorylation of isolated cardiac mitochondria (part 8). RESULTS
At the end of the experiment, CP reached an almost threefold higher value than that measured for ATP. The presence of free calcium (0.34 mM) in the incubation medium significantly modified the polarographic re-2″t” cording. The ADP-Mg complex stimulated the oxygen uptake, but at a lower rate than in the absence of free calcium. Interestingly, the oxygen consumption remained elevated and no return to state IV respiration rate occurred during the course of the experiment. The determination of high energy phosphates revealed an initial decrease in ADP followed after 2 mins by a stabilization at an elevated value corresponding to approximately 30% of the added quantity. ATP was produced but reached a plateau of 350 nmoles within the first 2 mins.AMP was detected in noticeable amounts (approximately 200 nmoles) and CP was produced regularly throughout the experiment. Interestingly, the amount of CP was higher in the presence of free calcium (P<0.001) than in the buffer devoid of free calcium. Increasing the free calcium concentration to 0.50 |jM further decreased the oxygen consumption rate (compared with 0.34 |mM of free calcium) but did not noticeably modify the amount in high energy phosphates in the medium. The rate of energy production was not affected by the presence of free calcium but the mitochondrial metabolic efficiency was strongly reduced (Figure 3). For this last parameter, no significant difference appeared between 0.34 and 0.5 |mM of free calcium. Dreaming of a reliable pharmacy that could give you an opportunity to buy any amounts of with no prescription required and spend less money?
Figure 3 Influence of dietary polyunsaturatedfatty acids on the rate of energy production and metabolic efficiency of isolated mitochondria oxidizing palmitoylcarnitine in buffers differing in their free calcium concentration (0, 0.34 and 0.50 цM, respectively). The rate of energy production and the mitochondrial metabolic efficiency were calculated as described in the materials and methods section. There were 10 animals per group. *P<0.01. FOFish oil group; SSO Sunflower seed oil group