Influence of the phospholipid n-6:n-3 polyunsaturated fatty acid ratio on the oxidative phosphorylation of isolated cardiac mitochondria (part 7). RESULTS

The mitochondrial oxygen uptake in states III and IV was influenced by the free calcium concentration in the medium (Table 3). In the absence of free calcium, a conventional polarographic recording was obtained. It was characterized by2_j_an ADP-Mg -induced stimulation of oxygen consumption (state III respiration rate) followed by a reduction of the respiration rate (state IV respiration rate). In these conditions,the added ADP-Mg was totally phosphorylated in ATP within the first 2 mins following the addition of the phosphorylation-stimulating substrate (Figure 1). The return to state IV respiration rate corresponded to the total ADP phosphorylation. AMP was not detected in noticeable amounts and CP was regularly produced throughout the 10 min incubation (Figure 2). Shop online with the best pharmacy that will ensure high quality of your medications and will offer cheapest with no rx required any time you need this or any other one for your medical problem.

Influence of the phospholipid n-6:n-3 polyunsaturated fatty acid ratio on the oxidative phosphorylation of isolated cardiac mitochondria

Figure 1 Production of adenine nucleotides by n-6 and n-3polyunsaturatedfatty acid-rich mitochondria oxidizingpalmitoylcarnitine in buffers differing in their free calcium concentration (0, 0.34 and 0.50 цM, respectively). There were 10 animals per group. FO Fish oil group; NS Not significant; SSO Sunflower seed oil group; ANOVA: O ef Oil effect; Ca efFree calcium effect; CI Cross-interaction between these two factors

Influence of the phospholipid n-6:n-3 polyunsaturated fatty acid ratio on the oxidative phosphorylation of isolated cardiac mitochondria

Figure 2 Production ofcreatine phosphate by n-6 and n-3 polyunsaturatedfatty acid-rich mitochondria oxidizing palmitoylcarnitine in buffers differing in their free calcium concentration (0, 0.34 and 0.50 цM, respectively). There were 10 animals per group. FOFish oil group; NSNot significant; SSO Sunflower seed oil group; ANOVA: O efOil effect; Ca~ efFree calcium effect; CI Cross-interaction between these two factors

TABLE 3 Influence of free calcium concentration and phospholipid polyunsaturated fatty acid composition on state III and state IV respiration rates and creatine phosphate (CP) production in isolated mitochondria oxidizing palmitoylcarnitine as a substrate

Ca2+ Respiration rates CP
concentration Diet State III State IV production
0 ^M FO 357±10.6 71 ±4.3 214+11.3
SSO 403±16.3 75±3.8 265±16.1
0.34 FO 181 ±11.1 181 ±11.1 260±16.9
SSO 261 ±10.5 261 ±10.5 273±13.4
0.50 ^M FO 170±10.6 170±10.6 222±16.7
SSO 221 ±15.3 221 ±15.3 243±17.1
ANOVA O ef 0.001 0.001 0.05
Ca2+ ef 0.001 0.001 NS
CI NS 0.01 NS

There were 10 animals per group. FO Fish oil; SSO Sunflower seed oil; ANOVA: O ef Oil effect; Ca

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